RAPID SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES ON A GRAFTED POLYMER SUPPORT

被引：57

作者：

POTAPOV, VK ^{[1
]}

VEIKO, VP ^{[1
]}

KOROLEVA, ON ^{[1
]}

SHABAROVA, ZA ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,BELOZERSKII MOLEC BIOL & BIOORGAN CHEM LAB,MOSCOW 117234,USSR

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 06期

关键词：

D O I：

10.1093/nar/6.6.2041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polyaterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 t o 15 nucleotides long.A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 μmol per gram of support. © 1979 Information Retrieval Limited.

引用

页码：2041 / 2056

页数：16

共 17 条

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