RAPID COLORIMETRIC DETECTION OF INVITRO AMPLIFIED DNA-SEQUENCES

被引：31

作者：

LUNDEBERG, J ^{[1
]}

WAHLBERG, J ^{[1
]}

HOLMBERG, M ^{[1
]}

PETTERSSON, U ^{[1
]}

UHLEN, M ^{[1
]}

机构：

[1] UNIV UPPSALA,DEPT MED GENET,S-75123 UPPSALA,SWEDEN

来源：

DNA AND CELL BIOLOGY | 1990年 / 9卷 / 04期

关键词：

D O I：

10.1089/dna.1990.9.287

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A colorimetric assay to detect immobilized amplified nucleic acids has been designed. This approach provides a rapid assay, suitable for clinical diagnosis, to analyze DNA sequences amplified by the polymerase chain reaction. The specific DNA sequences are captured on a solid support by the use of a recombinant fusion protein consisting of the Escherichia coli lac repressor and staphylococcal protein A. The biotin streptavidin system is used to detect the immobilized material. Positive samples can be analyzed by direct solid-phase sequencing. Here, we show that this nonradioactive concept can be used for analysis of Staphylococci and Streptococci and for specific detection of the protozoa Plasmodium falciparum in clinical samples. © 1990, Mary Ann Liebert, Inc. All rights reserved.

引用

页码：287 / 292

页数：6

共 20 条

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