SMALL INTRONS IN A HEPATIC CDNA-ENCODING A NEW GLUCAGON-LIKE PEPTIDE-1-TYPE RECEPTOR

被引：32

作者：

SVOBODA, M ^{[1
]}

CICCARELLI, E ^{[1
]}

TASTENOY, M ^{[1
]}

CAUVIN, A ^{[1
]}

STIEVENART, M ^{[1
]}

CHRISTOPHE, J ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,SCH MED,DEPT BIOCHEM & NUTR,BLDG GE CP 611,808 ROUTE LENNIK,B-1070 BRUSSELS,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 191卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA fragment was PCR amplified at low-stringency from rat DNA, using two degenerated primers corresponding to consensus sequences in the second and third transmembrane segments of members of the secretin receptor family. A synthetic oligodeoxynucleotide based on the 3′ end of this fragment, when used in Northern-blot analysis, hybridized to a broad 1.5 - 3.0 kb band in rat liver. It was then used as a probe for screening a cDNA library generated from size-fractionated rat liver poly(A)+ RNA. Overlapping alignment of sequences from 3 clones identified a 1455 residue open reading frame encoding a 485 amino acid peptide showing 7 putative transmembrane segments and 42 % identity with the glucagon-like peptide 1 (GLP-1) receptor. In addition, four small (85-300 bp) introns were variously represented in the isolated clones. © 1993 Academic Press, Inc.

引用

收藏

页码：479 / 486

页数：8

相关论文

共 14 条

[1] THE STRUCTURE OF NEUROPEPTIDE RECEPTORS [J].

BURBACH, JPH ;

MEIJER, OC .

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1992, 227 (01) :1-18

[2] MODEL SYSTEMS FOR THE STUDY OF 7-TRANSMEMBRANE-SEGMENT RECEPTORS [J].

DOHLMAN, HG ;

THORNER, J ;

CARON, MG ;

LEFKOWITZ, RJ .

ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :653-688

[3] FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF A NOVEL RECEPTOR FOR VASOACTIVE INTESTINAL POLYPEPTIDE [J].

ISHIHARA, T ;

SHIGEMOTO, R ;

MORI, K ;

TAKAHASHI, K ;

NAGATA, S .

NEURON, 1992, 8 (04) :811-819

[4] MOLECULAR-CLONING AND EXPRESSION OF A CDNA-ENCODING THE SECRETIN RECEPTOR [J].

ISHIHARA, T ;

NAKAMURA, S ;

KAZIRO, Y ;

TAKAHASHI, T ;

TAKAHASHI, K ;

NAGATA, S .

EMBO JOURNAL, 1991, 10 (07) :1635-1641

[5]

IYENGAR R, 1984, J BIOL CHEM, V259, P5222

[6] A G-PROTEIN LINKED RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID-HORMONE RELATED PEPTIDE [J].

JUPPNER, H ;

ABOUSAMRA, AB ;

FREEMAN, M ;

KONG, XF ;

SCHIPANI, E ;

RICHARDS, J ;

KOLAKOWSKI, LF ;

HOCK, J ;

POTTS, JT ;

KRONENBERG, HM ;

SEGRE, GV .

SCIENCE, 1991, 254 (5034) :1024-1026

[7] EXPRESSION CLONING OF AN ADENYLATE-CYCLASE COUPLED CALCITONIN RECEPTOR [J].

LIN, HY ;

HARRIS, TL ;

FLANNERY, MS ;

ARUFFO, A ;

KAJI, EH ;

GORN, A ;

KOLAKOWSKI, LF ;

LODISH, HF ;

GOLDRING, SR .

SCIENCE, 1991, 254 (5034) :1022-1024

[8]

ODOWD BF, 1989, J BIOL CHEM, V264, P7564

[9] SIGNALS FOR THE SELECTION OF A SPLICE SITE IN PRE-MESSENGER-RNA - COMPUTER-ANALYSIS OF SPLICE JUNCTION SEQUENCES AND LIKE SEQUENCES [J].

OHSHIMA, Y ;

GOTOH, Y .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (02) :247-259

[10] STRUCTURAL REQUIREMENTS FOR THE OCCUPANCY OF PITUITARY ADENYLATE-CYCLASE-ACTIVATING-PEPTIDE (PACAP) RECEPTORS AND ADENYLATE-CYCLASE ACTIVATION IN HUMAN NEUROBLASTOMA NB-OK-1 CELL-MEMBRANES - DISCOVERY OF PACAP(6-38) AS A POTENT ANTAGONIST [J].

ROBBERECHT, P ;

GOURLET, P ;

DENEEF, P ;

WOUSSENCOLLE, MC ;

VANDERMEERSPIRET, MC ;

VANDERMEERS, A ;

CHRISTOPHE, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 207 (01) :239-246