SMALL INTRONS IN A HEPATIC CDNA-ENCODING A NEW GLUCAGON-LIKE PEPTIDE-1-TYPE RECEPTOR

被引：32

作者：

SVOBODA, M ^{[1
]}

CICCARELLI, E ^{[1
]}

TASTENOY, M ^{[1
]}

CAUVIN, A ^{[1
]}

STIEVENART, M ^{[1
]}

CHRISTOPHE, J ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,SCH MED,DEPT BIOCHEM & NUTR,BLDG GE CP 611,808 ROUTE LENNIK,B-1070 BRUSSELS,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 191卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA fragment was PCR amplified at low-stringency from rat DNA, using two degenerated primers corresponding to consensus sequences in the second and third transmembrane segments of members of the secretin receptor family. A synthetic oligodeoxynucleotide based on the 3′ end of this fragment, when used in Northern-blot analysis, hybridized to a broad 1.5 - 3.0 kb band in rat liver. It was then used as a probe for screening a cDNA library generated from size-fractionated rat liver poly(A)+ RNA. Overlapping alignment of sequences from 3 clones identified a 1455 residue open reading frame encoding a 485 amino acid peptide showing 7 putative transmembrane segments and 42 % identity with the glucagon-like peptide 1 (GLP-1) receptor. In addition, four small (85-300 bp) introns were variously represented in the isolated clones. © 1993 Academic Press, Inc.

引用

页码：479 / 486

页数：8

共 14 条

[11] VALIDITY OF THE SUPERIORITY AND GOAL INSTABILITY SCALES AS PREDICTORS OF WOMENS ADJUSTMENT TO COLLEGE LIFE [J].