RADIOIMMUNOASSAY FOR HUMAN INSULIN-LIKE GROWTH FACTOR-I RECEPTOR - APPLICABILITY TO BREAST-CARCINOMA SPECIMENS AND CELL-LINES

被引：10

作者：

PEZZINO, V

MILAZZO, G

FRITTITTA, L

VIGNERI, R

EZAKI, O

KASAHARA, M

LEBON, TR

GOLDFINE, ID

FUJITAYAMAGUCHI, Y

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DEPT MOLEC GENET,1450 E DUARTE RD,DUARTE,CA 91010

[2] UNIV CATANIA,CATTEDRA ENDOCRINOL,I-95124 CATANIA,ITALY

[3] UNIV TOKYO,FAC SCI,DEPT BIOL,TOKYO 113,JAPAN

[4] UNIV CALIF SAN FRANCISCO,MT ZION HOSP,DIV DIABET & ENDOCRINE RES,SAN FRANCISCO,CA 94143

来源：

METABOLISM-CLINICAL AND EXPERIMENTAL | 1991年 / 40卷 / 08期

关键词：

D O I：

10.1016/0026-0495(91)90016-P

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A radioimmunoassay for the human insulin-like growth factor-I (IGF-I) receptor was developed using a rabbit polyclonal antibody to the human IGF-I receptor and a highly purified IGF-I receptor. The purified receptor was radiolabeled with 125I-Bolton-Hunter reagent. Over 18% of the radiolabeled receptor was immunoprecipitated with the polyclonal antireceptor antibody. Purified IGF-I receptor concentrations as low as 5ng 0.5mL inhibited the radiolabeled IGF-I receptor binding. Purified insulin receptor weakly inhibited this binding, while the ligand IGF-I did not show inhibition. The radioimmunoassay was applicable to the measurements of IGF-I receptors in the Triton X-100 extracts of various tissues and cells. Breast cancer tissues and cells showed detectable IGF-I receptors, which correlated with IGF-I ligand binding. Receptor content was measurable in placenta and IM-9 cells, but receptor content was not measurable in liver and muscle extracts. © 1991.

引用

页码：861 / 865

页数：5

共 23 条

[1] BLOCKADE OF THE TYPE-I SOMATOMEDIN RECEPTOR INHIBITS GROWTH OF HUMAN-BREAST CANCER-CELLS IN ATHYMIC MICE [J].

ARTEAGA, CL ;

KITTEN, LJ ;

CORONADO, EB ;

JACOBS, S ;

KULL, FC ;

ALLRED, DC ;

OSBORNE, CK .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (05) :1418-1423

[2]

CORONADO E, 1988, P AM ASSOC CANC RES, V29, P237

[3]

CULLEN KJ, 1990, CANCER RES, V50, P48

[4]

EZAKI O, 1986, J BIOL CHEM, V261, P3295

[5]

FURLANETTO RW, 1984, CANCER RES, V44, P2122

[6]

KULL FC, 1983, J BIOL CHEM, V258, P6561

[7]

LEBON TR, 1986, J BIOL CHEM, V261, P7685

[8]

LIPPMAN ME, 1985, CLIN RES, V33, P375

[9] GROWTH REGULATORY PEPTIDE PRODUCTION BY HUMAN-BREAST CARCINOMA-CELLS [J].

LIPPMAN, ME ;

DICKSON, RB ;

GELMANN, EP ;

ROSEN, N ;

KNABBE, C ;

BATES, S ;

BRONZERT, D ;

HUFF, K ;

KASID, A .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1988, 30 (1-6) :53-61

[10]

MOXHAM GP, 1989, J BIOL CHEM, V257, P5038

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