RADIOIMMUNOASSAY FOR HUMAN INSULIN-LIKE GROWTH FACTOR-I RECEPTOR - APPLICABILITY TO BREAST-CARCINOMA SPECIMENS AND CELL-LINES

被引：10

作者：

PEZZINO, V

MILAZZO, G

FRITTITTA, L

VIGNERI, R

EZAKI, O

KASAHARA, M

LEBON, TR

GOLDFINE, ID

FUJITAYAMAGUCHI, Y

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DEPT MOLEC GENET,1450 E DUARTE RD,DUARTE,CA 91010

[2] UNIV CATANIA,CATTEDRA ENDOCRINOL,I-95124 CATANIA,ITALY

[3] UNIV TOKYO,FAC SCI,DEPT BIOL,TOKYO 113,JAPAN

[4] UNIV CALIF SAN FRANCISCO,MT ZION HOSP,DIV DIABET & ENDOCRINE RES,SAN FRANCISCO,CA 94143

来源：

METABOLISM-CLINICAL AND EXPERIMENTAL | 1991年 / 40卷 / 08期

关键词：

D O I：

10.1016/0026-0495(91)90016-P

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A radioimmunoassay for the human insulin-like growth factor-I (IGF-I) receptor was developed using a rabbit polyclonal antibody to the human IGF-I receptor and a highly purified IGF-I receptor. The purified receptor was radiolabeled with 125I-Bolton-Hunter reagent. Over 18% of the radiolabeled receptor was immunoprecipitated with the polyclonal antireceptor antibody. Purified IGF-I receptor concentrations as low as 5ng 0.5mL inhibited the radiolabeled IGF-I receptor binding. Purified insulin receptor weakly inhibited this binding, while the ligand IGF-I did not show inhibition. The radioimmunoassay was applicable to the measurements of IGF-I receptors in the Triton X-100 extracts of various tissues and cells. Breast cancer tissues and cells showed detectable IGF-I receptors, which correlated with IGF-I ligand binding. Receptor content was measurable in placenta and IM-9 cells, but receptor content was not measurable in liver and muscle extracts. © 1991.

引用

页码：861 / 865

页数：5

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