A COOLING CHAIN FOR STUDIES OF CRYOFIXED BIOLOGICAL SPECIMENS BY SCANNING-TRANSMISSION ELECTRON-MICROSCOPY AND X-RAY-MICROANALYSIS

被引：26

作者：

ZIEROLD, K

KONIG, R

OLECH, KH

SCHAFER, D

LUBBERS, DW

MULLER, KH

WINTER, H

机构：

[1] Max Planck Inst. Systemphysiol., D-4600 Dortmund 1, Germany

来源：

ULTRAMICROSCOPY | 1981年 / 6卷 / 02期

关键词：

D O I：

10.1016/0304-3991(81)90058-9

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A cooling chain is described which enables the transfer of frozen hydrated biological specimens (ultrathin cryosections as well as about 1 mu m thick cultured cells) from a cryoultramicrotome into a scanning transmission electron microscope with a field emission gun. Transfer is done at 118 K, specimen temperature in the microscope is 165 K. Sublimation processes are controlled visually and by mass spectrometry. Electron micrographs and X-ray microanalysis spectra of cryofixed unstained tissue culture cells and rat liver tissue sections are described and discussed. Contamination of the specimen is much reduced by use of the cold stage.

引用

页码：181 / 186

页数：6

共 21 条

[1] DIRECT MEASUREMENT OF TEMPERATURE-CHANGES WITHIN FREEZE-FRACTURE SPECIMENS DURING RAPID QUENCHING IN LIQUID COOLANTS [J].