VOLTAGE-SENSITIVE ADENYLYL-CYCLASE ACTIVITY IN CULTURED NEURONS - A CALCIUM-INDEPENDENT PHENOMENON

被引：63

作者：

REDDY, R ^{[1
]}

SMITH, D ^{[1
]}

WAYMAN, G ^{[1
]}

WU, ZL ^{[1
]}

VILLACRES, EC ^{[1
]}

STORM, DR ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT PHARMACOL, SEATTLE, WA 98195 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 24期

关键词：

D O I：

10.1074/jbc.270.24.14340

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Catalytic subunits of mammalian adenylyl cyclases have been proposed to contain 12 transmembrane domains, a property shared with some voltage-sensitive ion channels. Here we report that adenylyl cyclase activity in cerebellar neurons is synergistically stimulated by depolarizing agents and beta-adrenergic receptor activation. This phenomenon is Ca2+-independent and not attributable to Ca2+-stimulated adenylyl cyclase activity. Cholera toxin and forskolin also synergistically stimulate adenylyl cyclase activity in combination with depolarizing agents. We hypothesize that conformational changes in the catalytic subunit of the enzymes caused by changes in the membrane potential may enhance stimulation of adenylyl cyclases by the guanylyl nucleotide stimulatory protein. This novel mechanism for regulation of adenylyl cyclases generates robust cAMP signals that may contribute to various neuromodulatory events including some forms of neuroplasticity.

引用

页码：14340 / 14346

页数：7

共 55 条

[1] SPATIALLY RESOLVED DYNAMICS OF CAMP AND PROTEIN KINASE-A SUBUNITS IN APLYSIA SENSORY NEURONS [J].