RAPID AGGLUTINATION TESTING IN AN ULTRASONIC STANDING-WAVE

被引：43

作者：

GRUNDY, MA

BOLEK, WE

COAKLEY, WT

BENES, E

机构：

[1] UNIV WALES COLL CARDIFF,COLL CARDIFF,SCH PURE & APPL BIOL,CATHAYS PK,CARDIFF CF1 3TL,WALES

[2] VIENNA TECH UNIV,INST ALLGEMEINE PHYS,A-1040 VIENNA,AUSTRIA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 165卷 / 01期

关键词：

ULTRASOUND; ULTRASONIC STANDING WAVE FIELD; AGGLUTINATION TEST; RING TRANSDUCER; IMMUNOPRECIPITATION;

D O I：

10.1016/0022-1759(93)90105-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The time taken to perform diagnostic agglutination tests can be significantly reduced by applying an ultrasonic standing wave field to a droplet of reactants held in a capillary tube. Avian erythrocytes, bacteria and latex particles from commercially available test kits were agglutinated in 15 s, 5 min, and 1 min respectively. These times compare favourably with the times of 30 min, 4 h, and 8 min required for agglutination by the methods prescribed for the respective kits. No loss in sensitivity or specificity was observed with the ultrasonic method. A multi-test procedure is also described whereby a series of five droplets loaded in a single capillary can be tested in less than 4 min by drawing the capillary along the axis of the ultrasonic field of a ring transducer.

引用

页码：47 / 57

页数：11