COMPARISON OF ARBITRARILY PRIMED PCR AND MACRORESTRICTION (PULSED-FIELD GEL-ELECTROPHORESIS) TYPING OF PSEUDOMONAS-AERUGINOSA STRAINS FROM CYSTIC-FIBROSIS PATIENTS

被引：71

作者：

KERSULYTE, D

STRUELENS, MJ

DEPLANO, A

BERG, DE

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110

[3] HOP ERASME,MICROBIOL LAB,B-1070 BRUSSELS,BELGIUM

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 08期

关键词：

D O I：

10.1128/JCM.33.8.2216-2219.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Arbitrarily primed PCR fingerprinting was carried out on 43 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients. Seventeen major groups of strains that coincided with groups also distinguished by macrorestriction (pulsed-field gel electrophoresis) typing were identified. Our results illustrated that a CF patient can carry more than one strain and can carry a given strain for long periods of time and that strains can evolve by changes in drug resistance or other phenotypic traits during long-term colonization. The arbitrarily primed PCR method is recommended for first-pass screening of P. aeruginosa isolates from CF patients, especially when many strains are to be typed, because of its sensitivity and efficiency.

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页码：2216 / 2219

页数：4

相关论文

共 21 条

[1] DNA DIVERSITY AMONG CLINICAL ISOLATES OF HELICOBACTER-PYLORI DETECTED BY PCR-BASED RAPD FINGERPRINTING [J].

AKOPYANZ, N ;

BUKANOV, NO ;

WESTBLOM, TU ;

KRESOVICH, S ;

BERG, DE .

NUCLEIC ACIDS RESEARCH, 1992, 20 (19) :5137-5142

[2]

Baltimore Robert S., 1993, P25

[3]

Berg Douglas E., 1994, Methods in Molecular and Cellular Biology, V5, P13

[4] MOLECULAR EPIDEMIOLOGIC ANALYSIS OF PSEUDOMONAS-AERUGINOSA STRAINS CAUSING FAILURE OF ANTIBIOTIC-THERAPY IN CYSTIC-FIBROSIS PATIENTS [J].

BINGEN, E ;

DENAMUR, E ;

PICARD, B ;

GOULLET, P ;

LAMBERTZECHOVSKY, N ;

FOUCAUD, P ;

NAVARRO, J ;

ELION, J .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1992, 11 (05) :432-437

[5] DNA-SEQUENCE DIVERGENCE AMONG DERIVATIVES OF ESCHERICHIA-COLI K-12 DETECTED BY ARBITRARY PRIMER PCR (RANDOM AMPLIFIED POLYMORPHIC DNA) FINGERPRINTING [J].

BRIKUN, I ;

SUZIEDELIS, K ;

BERG, DE .

JOURNAL OF BACTERIOLOGY, 1994, 176 (06) :1673-1682

[6] PSEUDOMONAS-AERUGINOSA CORNEAL ULCER ISOLATES DISTINGUISHED USING THE ARBITRARILY PRIMED PCR DNA-FINGERPRINTING METHOD [J].

BUKANOV, N ;

RAVI, VN ;

MILLER, D ;

SRIVASTAVA, K ;

BERG, DE .

CURRENT EYE RESEARCH, 1994, 13 (11) :783-790

[7] GENOME FINGERPRINTING OF PSEUDOMONAS-AERUGINOSA INDICATES COLONIZATION OF CYSTIC-FIBROSIS SIBLINGS WITH CLOSELY RELATED STRAINS [J].

GROTHUES, D ;

KOOPMANN, U ;

VONDERHARDT, H ;

TUMMLER, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (10) :1973-1977

[8] ANTIBIOTIC-THERAPY FOR CHRONIC INFECTION OF PSEUDOMONAS IN THE LUNG [J].

HOIBY, N .

ANNUAL REVIEW OF MEDICINE, 1993, 44 :1-10

[9] DIVERSITY AMONG CLINICAL ISOLATES OF HISTOPLASMA-CAPSULATUM DETECTED BY POLYMERASE CHAIN-REACTION WITH ARBITRARY PRIMERS [J].

KERSULYTE, D ;

WOODS, JP ;

KEATH, EJ ;

GOLDMAN, WE ;

BERG, DE .

JOURNAL OF BACTERIOLOGY, 1992, 174 (22) :7075-7079

[10] NONMOTILITY AND PHAGOCYTIC RESISTANCE OF PSEUDOMONAS-AERUGINOSA ISOLATES FROM CHRONICALLY COLONIZED PATIENTS WITH CYSTIC-FIBROSIS [J].

MAHENTHIRALINGAM, E ;

CAMPBELL, ME ;

SPEERT, DP .

INFECTION AND IMMUNITY, 1994, 62 (02) :596-605