FLUORESCENCE IN-SITU HYBRIDIZATION OF MULTIPLE PROBES ON A SINGLE MICROSCOPE SLIDE

被引：5

作者：

LARIN, Z

FRICKER, MD

MAHER, E

ISHIKAWABRUSH, Y

SOUTHERN, EM

机构：

[1] UNIV OXFORD,DEPT PLANT SCI,OXFORD OX1 3RB,ENGLAND

[2] CHURCHILL HOSP,OXFORD CYTOGENET LAB,OXFORD OX3 7LJ,ENGLAND

[3] JOHN RADCLIFFE HOSP,INST MOLEC MED,ICRF,HUMAN GENET LAB,OXFORD OX3 9DU,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 18期

关键词：

D O I：

10.1093/nar/22.18.3689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a method to analyse multiple samples by fluorescence in situ hybridisation on a single glass microscope slide. Wells were formed in which independent hybridisation reactions could proceed by sealing a silicon rubber gasket to the slide. In the largest format tested, different probes were hybridised simultaneously by applying them directly from a 96-well microtitre dish which was inverted on a glass plate. This technique will increase the rate of analysis of multiple probes against a standard set of chromosomes and could also be used to analyse different karyotypes using a panel of probes such as single chromosome paints during a single operation. It should be useful for both chromosomal mapping projects and screening for chromosome abnormalities in clinical diagnostic laboratories.

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收藏

页码：3689 / 3692

页数：4

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