HUMAN ONCOGENE OF THE RAS SUPERFAMILY UNMASKED BY EXPRESSION CDNA CLONING

被引:87
作者
CHAN, AML [1 ]
MIKI, T [1 ]
MEYERS, KA [1 ]
AARONSON, SA [1 ]
机构
[1] NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892
关键词
D O I
10.1073/pnas.91.16.7558
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
As an approach to identify human oncogenes, we generated an expression cDNA library from an ovarian carcinoma line. A potent transforming gene was detected by transfection analysis and identified as TC21, a recently cloned member of the RAS gene superfamily. A single point mutation substituting glutamine for leucine at position 72 was shown to be responsible for activation of transforming properties. While the cDNA clone possessed high transforming activity, the ovarian tumor genomic DNA, which contained the mutated TC21 allele, failed to induce transformed foci. Thus, expression cDNA cloning made it possible to identify and isolate a human oncogene that has evaded detection by conventional approaches.
引用
收藏
页码:7558 / 7562
页数:5
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