GLYCOPROTEINS FROM EXPERIMENTAL GRANULATION-TISSUE AND THEIR EFFECTS ON COLLAGEN-SYNTHESIS IN EMBRYONIC CHICK TENDON CELLS

被引：6

作者：

AALTO, M

POTILA, M

KULONEN, E

机构：

[1] Department of Medical Chemistry, University of Turku

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 587卷 / 04期

基金：

英国医学研究理事会;

关键词：

(Embryonic chick); Collagen synthesis; Glycoprotein; Granulation tissue; N-Acetylneuraminic acid; Tendon cell;

D O I：

10.1016/0304-4165(79)90012-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Buffer-soluble and pronase-liberated glycoproteins from experimental granulation tissue were fractionated by gel filtration and DEAE-cellulose chromatography. The age of the granuloma was reflected in the gel filtration pattern. Two glycoproteins were isolated, purified to homogeneity and analyzed for their carbohydrate and amino acid compositions. The collagen synthesis in embryonic chick tendon cells was measured in the presence of these fractions, which were found to be inhibiting even at 10-6 M. These glycoproteins may be significant in the feedback regulation of the development of granulation tissue and fibrosis. © 1979.

引用

页码：606 / 617

页数：12

共 44 条

[1] EFFECT OF NEURAMINIDASE ON SYNTHESIS OF COLLAGEN AND OTHER PROTEINS [J].

AALTO, M ;

RONNEMAA, T ;

KULONEN, E .

BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 342 (02) :247-253

[2] INHIBITION OF PROTEIN-SYNTHESIS IN TENDON CELLS BY EXTRACTS FROM EXPERIMENTAL GRANULATION TISSUE [J].

AALTO, M ;

KULONEN, E .

FEBS LETTERS, 1974, 49 (01) :70-72

[3] EFFECT OF SILICA-LIBERATED MACROPHAGE FACTORS ON PROTEIN-SYNTHESIS IN CELL-FREE SYSTEMS [J].

AHO, S ;

KULONEN, E .

EXPERIMENTAL CELL RESEARCH, 1977, 104 (01) :31-38

[4] METHODS FOR QUANTITATIVE ESTIMATION OF N-ACETYLNEURAMINIC ACID AND THEIR APPLICATION TO HYDROLYSATES OF SIALOMUCOIDS [J].

AMINOFF, D .

BIOCHEMICAL JOURNAL, 1961, 81 (02) :384-&

[5]

Anderson J C, 1976, Int Rev Connect Tissue Res, V7, P251

[6] IMPROVED SEPARATION OF SERUM PROTEINS IN PAPER ELECTROPHORESIS - A NEW ELECTROPHORESIS BUFFER [J].

ARONSSON, T ;

GRONWALL, A .

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1957, 9 (04) :338-341

[7] ISOLATION OF GLYCOPEPTIDES FROM LOW-DENSITY AND HIGH-DENSITY PLATELET PLASMA MEMBRANES [J].

BARBER, AJ ;

JAMIESON, GA .

BIOCHEMISTRY, 1971, 10 (25) :4711-+

[8]

BOLE GG, 1975, J LAB CLIN MED, V86, P1018

[9] SYNTHESIS AND EXTRUSION OF COLLAGEN BY FRESHLY ISOLATED CELLS FROM CHICK EMBRYO TENDON [J].

DEHM, P ;

PROCKOP, DJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 240 (03) :358-&

[10] ISOLATION AND CHARACTERIZATION OF ACIDIC STRUCTURAL GLYCOPROTEINS IN PULMONARY TISSUES [J].

FRANCIS, G ;

THOMAS, J .

BIOCHEMICAL JOURNAL, 1975, 145 (02) :299-304

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