HETEROLOGOUS TRANSPOSON TAGGING OF THE DRL1 LOCUS IN ARABIDOPSIS

被引:103
作者
BANCROFT, I
JONES, JDG
DEAN, C
机构
[1] AFRC,JOHN INNES CTR,INST PLANT SCI RES,CAMBRIDGE LAB,NORWICH NR4 7UJ,ENGLAND
[2] JOHN INNES CTR,SAINSBURY LAB,NORWICH NR4 7UH,ENGLAND
关键词
D O I
10.1105/tpc.5.6.631
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The development of heterologous transposon tagging systems has been an important objective for many laboratories. Here, we demonstrate the use of a Dissociation (Ds) derivative of the maize transposable element Activator (Ac) to tag the DRL1 locus of Arabidopsis. The drl1 mutant shows highly abnormal development with stunted roots, few root hairs, lanceolate leaves, and a highly enlarged, disorganized shoot apex that does not produce an inflorescence. The mutation was shown to be tightly linked to a transposed Ds, and somatic instability was observed in the presence of the transposase source. Some plants showing somatic reversion flowered and produced large numbers of wild-type progeny. These revertant progeny always inherited a DRL1 allele from which Ds had excised. Analysis of the changes in DNA sequence induced by the insertion and excision of the Ds element showed that they were typical of those induced by Ac and Ds in maize.
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收藏
页码:631 / 638
页数:8
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