RELAXATION DYNAMICS OF MYOGLOBIN IN SOLUTION

被引：125

作者：

TIAN, WD

SAGE, JT

SRAJER, V

CHAMPION, PM

机构：

[1] Department of Physics, Northeastern University, Boston

来源：

PHYSICAL REVIEW LETTERS | 1992年 / 68卷 / 03期

关键词：

D O I：

10.1103/PhysRevLett.68.408

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

The geminate rebinding kinetics of MbCO in solution at high temperature (260-300 K) shows nonexponential behavior (stretched, beta approximately 1/2) that may be related to nonequilibrium relaxation of the distal pocket. A two-pulse photolysis experiment, which probes a kinetically selected subpopulation, demonstrates that the nonexponential behavior arises from a fluctuationally averaged system and also reveals slow interconversion times for large-scale protein motions. Measurements as a function of temperature lead to a direct determination of the Arrhenius barrier at the heme (18 +/- 2 kJ/mole).

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页码：408 / 411

页数：4

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