THE USE OF CHYMOTRYPSIN/GALACTOSE TO TREAT SPERMATOZOA BOUND WITH ANTISPERM ANTIBODIES PRIOR TO INTRAUTERINE INSEMINATION

被引：38

作者：

BOLLENDORF, A ^{[1
]}

CHECK, JH ^{[1
]}

KATSOFF, D ^{[1
]}

FEDELE, A ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY, COOPER HOSP,MED CTR,ROBERT WOOD JOHNSON MED SCH, DEPT OBSTET & GYNECOL, CAMDEN, NJ 08103 USA

来源：

HUMAN REPRODUCTION | 1994年 / 9卷 / 03期

关键词：

ALBUMIN; ANTISPERM ANTIBODIES; CHYMOTRYPSIN; GALACTOSE; INTRAUTERINE INSEMINATION; PREGNANCY RATES;

D O I：

10.1093/oxfordjournals.humrep.a138532

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Anti-sperm antibodies in semen have been associated with a decrease in fertility potential. The question arises as to whether intra-uterine insemination (IUI) can improve pregnancy rates by merely allowing earlier capacitation and close timing to ovulation, or whether certain treatments of the spermatozoa add extra benefit. The study presented herein was designed to compare IUI using Percoll density separation with an albumin treatment versus chymotrypsin/galactose treatment. Sixteen patients were evaluated where IUI was randomized between both sperm treatments. Pregnancy rates/cycle were 25% (eight of 32) with chymotrypsin/galactose-treated spermatozoa compared to only 3% (one of 33) cycles with albumin-treated spermatozoa (P < 0.01). Since it has been reported that the proportions of spermatozoa showing immunobead binding for specific antibodies after chymotrypsin/galactose treatment remain unchanged, the exact mechanism for improvement is unknown; possibly chymotrypsin/galactose interferes with the function of the antibodies.

引用

页码：484 / 488

页数：5