HIGH-RESOLUTION VISUAL MAPPING OF STRETCHED DNA BY FLUORESCENT HYBRIDIZATION

被引：243

作者：

PARRA, I ^{[1
]}

WINDLE, B ^{[1
]}

机构：

[1] CANC THERAPY & RES CTR S TEXAS,8122 DATAPOINT DR,SAN ANTONIO,TX 78229

来源：

NATURE GENETICS | 1993年 / 5卷 / 01期

关键词：

D O I：

10.1038/ng0993-17

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a >200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.

引用

页码：17 / 21

页数：5

共 17 条

[1] AMPLIFIED N-MYC IN HUMAN NEURO-BLASTOMA CELLS IS OFTEN ARRANGED AS CLUSTERED TANDEM REPEATS OF DIFFERENTLY RECOMBINED DNA
AMLER, LC
SCHWAB, M
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) : 4903 - 4913
[2] BALLANNECHANTEL.B, 1992, CELL, V70, P1059
[3] RADIATION HYBRID MAPPING - A SOMATIC-CELL GENETIC METHOD FOR CONSTRUCTING HIGH-RESOLUTION MAPS OF MAMMALIAN CHROMOSOMES
COX, DR
BURMEISTER, M
PRICE, ER
KIM, S
MYERS, RM
[J]. SCIENCE, 1990, 250 (4978) : 245 - 250
[4] EVANS GA, 1987, METHOD ENZYMOL, V152, P604
[5] CHROMOSOMAL REGION OF THE CYSTIC-FIBROSIS GENE IN YEAST ARTIFICIAL CHROMOSOMES - A MODEL FOR HUMAN GENOME MAPPING
GREEN, ED
OLSON, MV
[J]. SCIENCE, 1990, 250 (4977) : 94 - 98
[6] HIGH-RESOLUTION MAPPING OF MAMMALIAN GENES BY INSITU HYBRIDIZATION TO FREE CHROMATIN
HENG, HHQ
SQUIRE, J
TSUI, LC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) : 9509 - 9513
[7] EXTENDING THE CAPABILITIES OF INTERPHASE CHROMATIN MAPPING
LAWRENCE, JB
CARTER, KC
GERDES, MJ
[J]. NATURE GENETICS, 1992, 2 (03) : 171 - 172
[8] INTERPHASE AND METAPHASE RESOLUTION OF DIFFERENT DISTANCES WITHIN THE HUMAN DYSTROPHIN GENE
LAWRENCE, JB
SINGER, RH
MCNEIL, JA
[J]. SCIENCE, 1990, 249 (4971) : 928 - 932
[9] HIGH-RESOLUTION MAPPING OF HUMAN CHROMOSOME-11 BY INSITU HYBRIDIZATION WITH COSMID CLONES
LICHTER, P
TANG, CJC
CALL, K
HERMANSON, G
EVANS, GA
HOUSMAN, D
WARD, DC
[J]. SCIENCE, 1990, 247 (4938) : 64 - 69
[10] ORGANIZATION AND GENESIS OF DIHYDROFOLATE-REDUCTASE AMPLICONS IN THE GENOME OF A METHOTREXATE-RESISTANT CHINESE-HAMSTER OVARY CELL-LINE
MA, C
LOONEY, JE
LEU, TH
HAMLIN, JL
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) : 2316 - 2327

← 1 2 →