HPLC DETERMINATION OF GLUTATHIONE AND L-CYSTEINE IN PHARMACEUTICALS AFTER DERIVATIZATION WITH ETHACRYNIC-ACID

被引：20

作者：

DIPIETRA, AM ^{[1
]}

GOTTI, R ^{[1
]}

BONAZZI, D ^{[1
]}

ANDRISANO, V ^{[1
]}

CAVRINI, V ^{[1
]}

机构：

[1] UNIV BOLOGNA,DIPARTIMENTO SCI FARMACEUT,VIA BELMELORO 6,I-40126 BOLOGNA,ITALY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 01期

关键词：

GLUTATHIONE; L-CYSTEINE; ETHACRYNIC ACID AND ITS METHYL ESTER; PRE-CHROMATOGRAPHIC DERIVATIZATION; LIQUID CHROMATOGRAPHY;

D O I：

10.1016/0731-7085(94)80015-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ethacrynic acid and its methyl ester are proposed as useful pre-chromatographic derivatization reagents for the HPLC analysis (UV detection) of reduced glutathione (GSH) and L-Cysteine. The optimum experimental conditions for the thiol derivatization, the removal of the excess reagent by liquid-liquid or solid-phase extraction and the reversed-phase chromatographic separations of the thiol adducts were investigated. The method was applied to the HPLC determination of GSH and L-cysteine in commercial formulations and proved to be suitable for the HPLC determination of oxidized glutathione (GSSG) after reduction to GSH using dithiothreitol (DTT).

引用

页码：91 / 98

页数：8

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