MUSCARINIC RECEPTORS IN THE CENTRAL NERVOUS-SYSTEM OF THE RAT .2. DISTRIBUTION OF BINDING OF [PROPYLBENZILYLCHOLINE-H-3 MUSTARD IN THE MIDBRAIN AND HINDBRAIN

The distribution of muscarinic receptors has been studied in the rat midbrain and hindbrain by counting silver grains in light microscope autoradiographs of the specific (atropine-sensitive) binding of [3H]propylbenztlylcholine mustard in cryostat sections. Of the 78 areas studied 6 had grain counts between 6 and 9 times the nonspecific level ('high'), and a further 15 had counts 4-6 times non-specific ('intermediate'). The basilar pontine nuclei and the ventral nuclei of the lateral lemniscus had high counts. Among the cranial nerve motor nuclei the facial and hypoglossal nuclei had high counts and the motor trigeminal nucleus and nucleus ambiguus had medium counts. The interpeduncular nucleus as a whole had low counts but there were two bands of intense staining on each side around the entry zone of the bundles of afferent cholinergic fibres from the habenula. Intermediate levels of binding occurred over the inferior colliculus and the superficial and intermediate grey layers of the superior colliculus. The molecular layer of the vestibulocerebellar vermis was distinctly labelled. © 1979.