SIMULTANEOUS DETECTION OF DEHYDROASCORBIC, ASCORBIC, AND SOME ORGANIC-ACIDS IN FRUITS AND VEGETABLES BY HPLC

被引：80

作者：

NISPEROSCARRIEDO, MO

BUSLIG, BS

SHAW, PE

机构：

[1] U.S. Citrus and Subtropical Products Laboratory, South Atlantic Area, Agricultural Research Service, U.S.Department of Agriculture, Winter Haven, Florida 33883-1909, 600 Avenue S N.W.

[2] Florida Department of Citrus, Winter Haven, FL

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1992年 / 40卷 / 07期

关键词：

D O I：

10.1021/jf00019a007

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A high-performance liquid chromatography method was developed for the simultaneous determination of dehydroascrobic, ascorbic, malic, citric, and oxalic acids in fruits, vegetables, and beverages. Separation of these compounds was accomplished by coupling reversed-phase and organic acid columns using 2% KH2PO4 (pH 2.3) as mobile phase with a flow rate of 0.4 mL/min. Detection was performed at 215 and 260 nm using a diode array detector interfaced with portable integrators and a chromatography data system. Selected fresh fruits, vegetables, and commercial orange juices were analyzed using this method.

引用

页码：1127 / 1130

页数：4

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