STRUCTURE OF AN INTERFERON-ALPHA-2 GENE EXPRESSED IN THE BOVINE CONCEPTUS EARLY IN GESTATION

被引：39

作者：

STEWART, HJ ^{[1
]}

MCCANN, SHE ^{[1
]}

FLINT, APF ^{[1
]}

机构：

[1] AFRC,INST ZOOL,HORMONES & FARM ANIM REPROD RES GRP,LONDON NW1 4RY,ENGLAND

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 1990年 / 4卷 / 03期

关键词：

D O I：

10.1677/jme.0.0040275

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Genomic and cDNA clones for bovine trophoblast interferon (IFN) have been isolated by probing a bovine genomic library and a bovine embryonic (day-18 post coitus) cDNA library respectively with the ovine trophoblast IFN cDNA. The two DNA sequences were identical; sequence analysis demonstrated 80% identity between the amino acid sequence of bovine trophoblast IFN and ovine trophoblast IFN, and 70% identity with a previously identified bovine IFN-α2. Southern blotting of bovine genomic DNA indicated the presence of a minimum of three trophoblast IFN genes. Primer extension analysis identified the transcription start site in the 5' flanking region of the bovine IFN gene. Computer-aided analysis of the 5' flanking sequence demonstrated a similarity with that of bovine IFN-α2 and the existence of a possible viral induction sequence.

引用

页码：275 / 282

页数：8

共 25 条

[1] SYNTHESIS AND PROCESSING OF OVINE TROPHOBLAST PROTEIN-1 AND BOVINE TROPHOBLAST PROTEIN-1, CONCEPTUS SECRETORY PROTEINS INVOLVED IN THE MATERNAL RECOGNITION OF PREGNANCY [J].

ANTHONY, RV ;

HELMER, SD ;

SHARIF, SF ;

ROBERTS, RM ;

HANSEN, PJ ;

THATCHER, WW ;

BAZER, FW .

ENDOCRINOLOGY, 1988, 123 (03) :1274-1280

[2] CHARACTERIZATION OF PROTEINS PRODUCED INVITRO BY PERIATTACHMENT BOVINE CONCEPTUSES [J].

BARTOL, FF ;

ROBERTS, RM ;

BAZER, FW ;

LEWIS, GS ;

GODKIN, JD ;

THATCHER, WW .

BIOLOGY OF REPRODUCTION, 1985, 32 (03) :681-693

[3]

BAZER FW, 1986, J REPROD FERTIL, V76, P841, DOI 10.1530/jrf.0.0760841

[4]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[5] 2 DISTINCT FAMILIES OF HUMAN AND BOVINE INTERFERON-ALPHA GENES ARE COORDINATELY EXPRESSED AND ENCODE FUNCTIONAL POLYPEPTIDES [J].

CAPON, DJ ;

SHEPARD, HM ;

GOEDDEL, DV .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (04) :768-779

[6] CLONING AND EXPRESSION OF CDNA-ENCODING OVINE TROPHOBLASTIN - ITS IDENTITY WITH A CLASS-II ALPHA-INTERFERON [J].

CHARLIER, M ;

HUE, D ;

MARTAL, J ;

GAYE, P .

GENE, 1989, 77 (02) :341-348

[7] HIGH HOMOLOGY BETWEEN A TROPHOBLASTIC PROTEIN (TROPHOBLASTIN) ISOLATED FROM OVINE EMBRYO AND ALPHA-INTERFERONS [J].

CHARPIGNY, G ;

REINAUD, P ;

HUET, JC ;

GUILLOMOT, M ;

CHARLIER, M ;

PERNOLLET, JC ;

MARTAL, J .

FEBS LETTERS, 1988, 228 (01) :12-16

[8] COOPERATIVE INTERACTION OF MULTIPLE DNA ELEMENTS IN THE HUMAN INTERFERON-BETA PROMOTER [J].

DINTER, H ;

HAUSER, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (01) :103-109

[9] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[10] INTERFERON-BETA GENE-REGULATION - TANDEMLY REPEATED SEQUENCES OF A SYNTHETIC 6-BP OLIGOMER FUNCTION AS A VIRUS-INDUCIBLE ENHANCER [J].

FUJITA, T ;

SHIBUYA, H ;

HOTTA, H ;

YAMANISHI, K ;

TANIGUCHI, T .

CELL, 1987, 49 (03) :357-367

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