STRUCTURE OF AN INTERFERON-ALPHA-2 GENE EXPRESSED IN THE BOVINE CONCEPTUS EARLY IN GESTATION

被引：39

作者：

STEWART, HJ ^{[1
]}

MCCANN, SHE ^{[1
]}

FLINT, APF ^{[1
]}

机构：

[1] AFRC,INST ZOOL,HORMONES & FARM ANIM REPROD RES GRP,LONDON NW1 4RY,ENGLAND

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 1990年 / 4卷 / 03期

关键词：

D O I：

10.1677/jme.0.0040275

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Genomic and cDNA clones for bovine trophoblast interferon (IFN) have been isolated by probing a bovine genomic library and a bovine embryonic (day-18 post coitus) cDNA library respectively with the ovine trophoblast IFN cDNA. The two DNA sequences were identical; sequence analysis demonstrated 80% identity between the amino acid sequence of bovine trophoblast IFN and ovine trophoblast IFN, and 70% identity with a previously identified bovine IFN-α2. Southern blotting of bovine genomic DNA indicated the presence of a minimum of three trophoblast IFN genes. Primer extension analysis identified the transcription start site in the 5' flanking region of the bovine IFN gene. Computer-aided analysis of the 5' flanking sequence demonstrated a similarity with that of bovine IFN-α2 and the existence of a possible viral induction sequence.

引用

页码：275 / 282

页数：8

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