THE NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF THE RNA-1 SEGMENT IN 2 BROMOVIRUSES - BROAD BEAN MOTTLE VIRUS AND COWPEA CHLOROTIC MOTTLE VIRUS

被引:44
作者
DZIANOTT, AM
BUJARSKI, JJ
机构
[1] NO ILLINOIS UNIV,DEPT BIOL SCI,DE KALB,IL 60115
[2] NO ILLINOIS UNIV,CTR PLANT MOLEC BIOL,DE KALB,IL 60115
关键词
D O I
10.1016/0042-6822(91)90525-G
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The complete nucleotide sequences of the RNA-1 segments in broad bean mottle virus (BBMV) and cowpea chlorotic mottle virus (CCMV) were determined. BBMV RNA-1 consists of 3158 nucleotides and CCMV RNA-1 has 3171 nucleotides. Both BBMV and CCMV RNA-1 are capped at the 5′ end but, unlike in other tricornaviruses, BBMV RNA-1 initiates with an A residue. Both BBMV and CCMV RNA-1 are monocistronic encoding for highly homologous 1 a proteins of 966 and 958 amino acids, respectively. The highest homologies are clustered within two domains: the N-domain that aligns with the nsP1 Sindbis virus protein, a putative methyl transferase, and the C-domain which has a conserved nucleotide binding motif. Previous sequence comparisons suggest that the C-terminal domain may function as an NTP-dependent RNA helicase. In addition, we find that the C-domain has patterns similar to those of the reovirus and vaccinia virus guanylyl transferases. All this implies that 1 a protein is a multifunctional polypeptide involved in both RNA capping and RNA polymerization processes. © 1991.
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页码:553 / 562
页数:10
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