NEW ANTIGENS IN CELLS TRANSFORMED BY SV40 VIRUS .2. EVIDENCE OF THEIR CYTOPLASMIC LOCALIZATION IN A CELL LINE (TSV5 CL2) OF HAMSTER FIBROBLASTS TRANSFORMED BY SV40

A clone of hamster fibroblasts transformed by SV40 virus (TSV5 CL2) induced antibodies in hamsters against new antigenic constituents not present in the untransformed fibroblasts. The antibodies in the immune sera against histocompatibility antigens and those against nuclear T antigen were absorbed. The absorbed sera contained antibodies which agglutinated the transformed cells. The sera contained also precipitating antibodies against new antigens present in a crude extract of TSV5 CL2. These antibodies were directed to common new antigens present in SV40‐transformed fibroblasts from different species. Immunofluorescence and the “indirect enzyme‐labelled antibody technique” were used for the cellular localization of the antigens corresponding to the precipitating antibodies. Both techniques showed that these antigens were localized in the cytoplasm, mostly in the microsome‐ribosomal fractions, much less on the cell membrane and not at all in the nucleus. Copyright © 1969 Wiley‐Liss, Inc., A Wiley Company