Encapsulation and Reactivity of the Enzyme Oxalate Oxidase in a Sol-Gel Derived Glass

The enzymes oxalate oxidase and peroxidase are encapsulated in stable, optically transparent, porous silica glass matrices synthesized under mild conditions using novel sol-gel synthetic techniques. The large enzymes are immobilized, but smaller molecules such as oxalate ions pass readily through the porous glass. Upon exposure to oxalate solutions, a colored glass is formed whose absorption spectrum and changes of absorbance with time are measured. The sensitivity of the response and the time-dependence of the response are discussed.