Encapsulation and Reactivity of the Enzyme Oxalate Oxidase in a Sol-Gel Derived Glass

被引：5

作者：

Yamanaka, Stacey A. ^{[1
,2
]}

Nguyen, Nhi P. ^{[1
,2
]}

Ellerby, Lisa M. ^{[1
,2
]}

Dunn, Bruce ^{[1
,2
]}

Valentine, Joan S. ^{[1
,2
]}

Zink, Jeffrey I. ^{[1
,2
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, 405 Hilgard Ave, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, Dept Mat Sci & Engn, Los Angeles, CA 90024 USA

来源：

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY | 1994年 / 2卷 / 1-3期

基金：

美国国家科学基金会;

关键词：

biomaterials; sensors; enzyme; biosensors; spectroscopy in gels;

D O I：

10.1007/BF00486359

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The enzymes oxalate oxidase and peroxidase are encapsulated in stable, optically transparent, porous silica glass matrices synthesized under mild conditions using novel sol-gel synthetic techniques. The large enzymes are immobilized, but smaller molecules such as oxalate ions pass readily through the porous glass. Upon exposure to oxalate solutions, a colored glass is formed whose absorption spectrum and changes of absorbance with time are measured. The sensitivity of the response and the time-dependence of the response are discussed.

引用

页码：827 / 829

页数：3