STABILIZATION OF A CELL BIOCATALYST WITH BETA-GALACTOSIDASE ACTIVITY BY GLUTARALDEHYDE TREATMENT

被引：13

作者：

FLORES, MV

VOGET, CE

ERTOLA, RJJ

机构：

[1] NATL UNIV LA PLATA, FAC CIENCIAS EXACTAS, CTR INVEST & DESARROLLO FERMENTAC IND, RA-1900 LA PLATA, ARGENTINA

[2] UNIV MAR DEL PLATA, FAC INGN, INGN BIOQUIM GRP, RA-7600 MAR DEL PLATA, ARGENTINA

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1995年 / 64卷 / 04期

关键词：

KLUYVEROMYCES LACTIS; BETA-GALACTOSIDASE; WHOLE CELL BIOCATALYST; GLUTARALDEHYDE TREATMENT;

D O I：

10.1002/jctb.280640407

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study the stabilization with glutaraldehyde of permeabilized, non-viable Kluyveromyces lactis cells with beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) activity has been investigated. The influence of various factors in relation to the glutaraldehyde treatment, such as reaction time, temperature and glutaraldehyde and cell concentration, has been considered. The cell biocatalyst showed a good storage and operational stability. The kinetic properties of the biocatalyst in connection with lactose hydrolysis were found to be comparable to the cell-free enzyme.

引用

页码：353 / 360

页数：8

共 30 条

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