RECONSTITUTION OF MEMBRANES AND EMBRYONIC-DEVELOPMENT IN DISSOCIATED BLASTULA CELLS OF THE SEA-URCHIN BY RE-INSERTION OF AGGREGATION-PROMOTING MEMBRANE PROTEINS EXTRACTED WITH BUTANOL

被引：64

作者：

NOLL, H ^{[1
]}

MATRANGA, V ^{[1
]}

CASCINO, D ^{[1
]}

VITTORELLI, L ^{[1
]}

机构：

[1] IST ANAT COMPARATA,I-90123 PALERMO,ITALY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 01期

关键词：

D O I：

10.1073/pnas.76.1.288

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Blastula embryos of the sea urchin Paracentrotus lividus, when dissociated into single cells by exposure to Ca2+- and Mg2+-free sea water, reassociate spontaneously to form aggregates capable of development to the final larval form (pluteus). This aggregation is prevented by Fab fragments obtained by immunization with purified membranes from blastula embryos. The inhibition was reversed by soluble proteins extracted with butanol from purified membranes or from intact cells. These extracts also strongly stimulated the rate of reaggregation of dissociated cells in the absence of Fab fragments. Exposure of dissociated cells to 2.5% (vol/vol) butanol removed completely the protein(s) responsible for reaggregation of the cells without impairing their viability. Reaggregaton and embryonic development were completely restored to the extracted cells by readdition of the proteins extracted from either membranes or cells. Extracted cells from Paracentrotus could be reconstituted with proteins from Arbacia.

引用

页码：288 / 292

页数：5

共 26 条

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