CONSTRUCTION AND CHARACTERIZATION OF A MUTANT OF RHODOBACTER-SPHAEROIDES WITH THE REACTION CENTER AS THE SOLE PIGMENT PROTEIN COMPLEX

被引：98

作者：

JONES, MR ^{[1
]}

VISSCHERS, RW ^{[1
]}

VANGRONDELLE, R ^{[1
]}

HUNTER, CN ^{[1
]}

机构：

[1] FREE UNIV AMSTERDAM,FAC PHYS & ASTRON,DEPT BIOPHYS,1081 HV AMSTERDAM,NETHERLANDS

来源：

BIOCHEMISTRY | 1992年 / 31卷 / 18期

关键词：

D O I：

10.1021/bi00133a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A strain of Rhodobacter sphaeroides has been constructed in which the photosynthetic reaction center is the sole pigment-protein complex. The strain, named RCO1, is capable of photoheterotrophic growth and possesses assembled and functional reaction centers which can undergo photochemical charge separation and are reduced by electrons derived from the cytochrome b/c1 complex. The circular dichroism and linear dichroism of reaction centers in membranes from strain RCO1 are similar to those described previously for reaction centers isolated in detergent solution. A second strain, named RCLH11, which is devoid of the peripheral LH2 antenna complex has also been constructed. A description of the properties of these strains is presented.

引用

页码：4458 / 4465

页数：8

共 49 条

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