CITRATE SUBSTITUTES FOR HOMOCITRATE IN NITROGENASE OF A NIFV MUTANT OF KLEBSIELLA-PNEUMONIAE

被引：87

作者：

LIANG, JH

MADDEN, M

SHAH, VK

BURRIS, RH

机构：

[1] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706

[2] UNIV WISCONSIN,COLL AGR & LIFE SCI,CTR STUDY NITROGEN FIXAT,MADISON,WI 53706

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 37期

关键词：

D O I：

10.1021/bi00489a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An organic acid extracted from purified dinitrogenase isolated from a nifV mutant of Klebsiella pneumoniae has been identified as citric acid. H2 evolution by the citrate-containing dinitrogenase is partially inhibited by CO, and by some substrates for nitrogenase. The response of maximum velocities to changes in pH for both the wild-type and the NifV− dinitrogenase was compared. No substantial differences between the enzymes were observed, but there are minor differences. Both enzymes are stable in the pH range 4.8-10, but the enzyme activities dropped dramatically below pH 6.2.© 1990, American Chemical Society. All rights reserved.

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页码：8577 / 8581

页数：5

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