CHIRAL SEPARATION ON ACHIRAL STATIONARY PHASES WITH DIFFERENT FUNCTIONALITIES USING BETA-CYCLODEXTRIN IN THE MOBILE PHASE AND APPLICATION TO BIOANALYSIS AND COUPLED COLUMNS

Beta-cyclodextrin was used in the mobile phase as chiral selector for separating the enantiomers of terbutaline, chlorthalidone and oxazepam. The effect on chiral resolution using e.g. hydrophobic, polar or cation exchanging stationary phases was investigated. Both the chiral separation factor and retention level were affected by the concentration of methanol and beta-cyclodextrin. The stationary phase had no effect on the chiral separation only on the level of retention. By tuning the concentration of beta-cyclodextrin and methanol in the mobile phase chiral separation could be obtained on most stationary phases. By changing the stationary phase while adjusting the mobile phase composition to maintain the chiral selectivity improvements of the selectivity towards e.g. endogenous compounds can be obtained when separating enantiomers in complex matrixes as biological fluids. Further improvement on selectivity can be obtained if coupled columns are used. This is examplified for separation of chlorthalidone and terbutaline enantiomers in biological fluids by coupling an achiral column to another achiral column and using a mobile phase containing beta-cyclodextrin on the last column.