DEVELOPMENT OF POLYMERASE CHAIN-REACTION PRIMERS TO DETECT MYCOPLASMA-HYOPNEUMONIAE

被引:36
作者
ARTIUSHIN, S
STIPKOVITS, L
MINION, FC
机构
[1] IOWA STATE UNIV SCI & TECHNOL,INST VET MED RES,AMES,IA 50011
[2] HUNGARIAN ACAD SCI,INST VET MED RES,BUDAPEST,HUNGARY
关键词
POLYMERASE CHAIN REACTION; MYCOPLASMA HYOPNEUMONIAE;
D O I
10.1006/mcpr.1993.1056
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Mycoplasma hyopneumoniae (Mhp) is the etiologic agent of mycoplasma pneumonia in swine. The purpose of this study was to develop a species-specific polymerase chain reaction (PCR)-based diagnostic reagent for the identification of Mhp. Based upon DNA sequence analysis of a cloned fragment of Mhp DNA, PCR primers were constructed and tested against different strains of Mhp, Mycoplasma flocculare, other mycoplasma species, and non-Mollicute micro-organisms which commonly inhabit the respiratory tracts of swine. A total of 40 field isolates from Mhp and four field isolates of M. flocculare have been examined. Positive signals were obtained in PCR with Mhp reference strains and all 40 Mhp field isolates, but not with other Mollicutes or micro-organisms. © 1993 Academic Press Limited.
引用
收藏
页码:381 / 385
页数:5
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