DETECTION AND IDENTIFICATION OF MYCOPLASMAS BY AMPLIFICATION OF RDNA

被引：36

作者：

BLANCHARD, A ^{[1
]}

GAUTIER, M ^{[1
]}

MAYAU, V ^{[1
]}

机构：

[1] ECOLE NATL SUPER AGRON, RENNES, FRANCE

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 81卷 / 01期

关键词：

MYCOPLASMA SPP; POLYMERASE CHAIN REACTION; RDNA;

D O I：

10.1016/0378-1097(91)90467-O

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Alignment of published 16S rRNA sequences allowed the definition of a pair of oligonucleotides suitable for polymerase chain reaction (PCR). Using this pair of PCR primers, several mycoplasmas including the four human parasites Mycoplasma genitalium, M. hominis, M. salivarium and M. orale were detected. This DNA amplification was restricted to species of the genus Mycoplasma while no cross-reaction was observed with DNA from other bacteria and eukaryotic cells. Subsequent analysis of amplified products by either specific oligonucleotide hybridization or dideoxy sequencing specified the identity of the detected mycoplasmas. This method offers a highly discriminating and sensitive assay for the direct detection and identification of these microorganisms without the need for prior cultivation.

引用

页码：37 / 42

页数：6

共 18 条

[1] DETECTION OF MYCOPLASMA-PNEUMONIAE BY USING THE POLYMERASE CHAIN-REACTION [J].