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EXPRESSION OF THE SYMBIOTIC PLASMID FROM RHIZOBIUM-LEGUMINOSARUM BIOVAR TRIFOLII IN SPHINGOBACTERIUM-MULTIVORUM
被引:11
作者:
FENTON, M
[1
]
JARVIS, BDW
[1
]
机构:
[1] MASSEY UNIV, DEPT GENET & MICROBIOL, PALMERSTON NORTH, NEW ZEALAND
关键词:
RHIZOBIUM;
SPHINGOBACTERIUM;
NODULATION;
NOD GENE TRANSFER;
D O I:
10.1139/m94-138
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
An inoculant strain of Rhizobium leguminosarum biovar trifolii containing a Tn5 marked symbiotic plasmid transferred this plasmid by conjugation to Sphingobacterium multivorum, an organism that can be found in soil. The transconjugant bacteria nodulated the roots of white clover (Trifolium repens) seedlings but did not fix atmospheric nitrogen. Microscopic examination revealed abnormal nodule structures. Bacteria isolated from the nodules were shown to be closely related to the recipient S. multivorum and Southern blots of genomic digests probed with nodA DNA confirmed that the transconjugants contained symbiotic genes. This is the first report of the spontaneous transfer, by conjugation, of a symbiotic plasmid from R. leguminosarum biovar trifolii to S. multivorum.
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页码:873 / 879
页数:7
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