INHIBITORY EFFECT OF ANGIOTENSIN-II ON RENAL TUBULAR TRANSPORT

被引:36
作者
CHATSUDTHIPONG, V [1 ]
CHAN, YL [1 ]
机构
[1] UNIV ILLINOIS,COLL MED,DEPT PHYSIOL & BIOPHYS,CHICAGO,IL 60680
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 260卷 / 03期
关键词
BICARBONATE ABSORPTION; PROXIMAL CONVOLUTE TUBULE; ROLE OF CALCIUM; CAPILLARY MICROPERFUSION;
D O I
10.1152/ajprenal.1991.260.3.F340
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
This study was designed to examine the intracellular mechanism of inhibitory action of high concentrations of angiotensin II (ANG II) on proximal tubular transport in rat kidneys by microperfusion methods. Perfusion of ANG II (10(-6) M) to peritubular capillaries caused a reduction of both fluid and HCO3- transport (J(v) and J(HCO3-), respectively) by 33 and 26%, respectively. These inhibitory effects were blocked by the ANG II-receptor antagonist [Sar1,Ile8]ANG II (10(-5) M). Similar degrees of inhibition on J(v) and J(HCO3-) were observed when ionomycin (10(-7) and 10(-6) M), a Ca2+ ionophore, was added to capillary perfusate. Moreover, there was no additive effect when both ANG II and ionomycin were perfused together through capillaries, suggesting that both agents work via the same mechanism, presumably by increasing cytosolic Ca2+ concentration ([Ca2+]i). Inhibitory effects of ANG II on proximal tubular transport were still observed in a Ca2+ -free perfusate containing ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, indicating that these effects do not require influx of Ca2+ from extracellular medium. Furthermore, the observation that TMB-8, an agent that prevents intracellular Ca2+ mobilization, completely eliminated the effect of ANG II strongly suggests that intracellular Ca2+ rather than Ca2+ influx mediates effects of ANG II on proximal tubular transport. Direct measurement of [Ca2+]i by use of fura-2 in isolated proximal tubular cells showed slight but statistically significant increases in [Ca2+]i. Taken together, these observations support the idea that intracellular Ca2+ serves as a second messenger in the inhibitory effect of high concentrations of ANG II on J(v) and J(HCO3-) in proximal tubule of kidney.
引用
收藏
页码:F340 / F346
页数:7
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