CHOLESTEROL SENSOR-BASED ON ELECTRODEPOSITION OF CATALYTIC PALLADIUM PARTICLES

被引：33

作者：

DONG, SJ

DENG, Q

CHENG, GG

机构：

[1] Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 279卷 / 02期

关键词：

AMPEROMETRY; BIOSENSORS; CHOLESTEROL; ENZYME ELECTRODES; PALLADIUM;

D O I：

10.1016/0003-2670(93)80322-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A layer of palladium particles was electrodeposited on a glassy carbon electrode. The dispersed Pd particles resulted in a large decrease in overvoltage for the electrochemical oxidation of H2O2 down to +0.4 V vs. Ag/AgCl, based on which a new kind of cholesterol sensor was fabricated. Cholesterol oxidase was immobilized on the Pd-dispersed electrode by cross-linking with glutaraldehyde and a layer of poly(o-phenylenediamine) (PPD) film was electropolymerized on the enzyme layer. The sensor shows a linear response in the concentration range 0.05-4.50 mmol l-1 with a rapid response of less than 20 s. The polymer film can prevent interference from uric acid and ascorbic acid and also increases the thermal stability of the sensor. The sensor can be used for 200 assays without an obvious decrease in activity.

引用

页码：235 / 240

页数：6

共 21 条

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