ISOLATION AND H-1-NMR SPECTROSCOPIC IDENTIFICATION OF POLY(3-HYDROXYBUTANOATE) FROM PROKARYOTIC AND EUKARYOTIC ORGANISMS - DETERMINATION OF THE ABSOLUTE-CONFIGURATION (R) OF THE MONOMERIC UNIT 3-HYDROXYBUTANOIC ACID FROM ESCHERICHIA-COLI AND SPINACH

被引:75
作者
SEEBACH, D
BRUNNER, A
BURGER, HM
SCHNEIDER, J
REUSCH, RN
机构
[1] UNIV ZURICH, DEPT PATHOL, CH-8006 ZURICH, SWITZERLAND
[2] MICHIGAN STATE UNIV, DEPT MICROBIOL, E LANSING, MI 48824 USA
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 224卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1994.00317.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Trace amounts of poly[(R)-3-hydroxybutanoate] were isolated from competent Escherichia coli, spinach, bovine serum albumin, beef heart mitochondria, and aortal tissues, all sources in which it is not accumulated as storage material. Its identity was in all cases proved by H-1-NMR spectroscopy. In some runs, the poly[(R)-3-hydroxybutanoate] isolated from competent E. coli also contained some 3-hydroxyvalerate, an observation confirmed by H-1-NMR spectroscopy and gas chromatography. The absolute configuration of the polymers isolated from E. coli and spinach was shown to be (all-R) by gas chromatography on chiral columns.
引用
收藏
页码:317 / 328
页数:12
相关论文
共 44 条
[41]   CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE ALCALIGENES-EUTROPHUS H16 POLY-BETA-HYDROXYBUTYRATE BIOSYNTHETIC-PATHWAY [J].
SLATER, SC ;
VOIGE, WH ;
DENNIS, DE .
JOURNAL OF BACTERIOLOGY, 1988, 170 (10) :4431-4436
[42]  
STEINBUCHEL A, 1992, APPL MICROBIOL BIOT, V37, P1
[43]  
YALPANI M, 1992, Patent No. 9209210
[44]  
1994, BIOPOL NATURAL THERM