FEMTOMOLE OLIGOSACCHARIDE DETECTION USING A REDUCING-END DERIVATIVE AND CHEMICAL IONIZATION MASS-SPECTROMETRY

被引：17

作者：

CAESAR, JP

SHEELEY, DM

REINHOLD, VN

机构：

[1] Harvard University School of Public Health, Boston, MA 02115

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 191卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90215-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A bimodal reagent (pentafluorobenzyl aminobenzoate) has been synthesized to improve oligosaccharide isolation, detection, and structural characterization. The reagent is glycosidically attached to the reducing end of glycan residues, imparts fluorescent and uv properties for chromatographic detection, and functions as an efficient electron trap under negative ion chemical ionization mass spectrometry for femtomole detectability. Facile ester cleavage and pentafluorobenzyl elimination provides a single molecular-weight-related fragment in high abundance. Procedures are described for reagent synthesis, purification, and oligosaccharide conjugation. Carbohydrate samples derivatized with this reagent are evaluated by high-performance liquid chromatography and supercritical fluid chromatography (SFC) and for sensitivity by SFC negative ion chemical ionization mass spectrometry. © 1990.

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页码：247 / 252

页数：6

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