REQUIREMENT FOR LYMPHOCYTES AND RESIDENT MACROPHAGES IN LPS-INDUCED PLEURAL EOSINOPHIL ACCUMULATION

被引:24
作者
BOZZA, PT [1 ]
CASTROFARIANETO, HC [1 ]
PENIDO, C [1 ]
LARANGEIRA, AP [1 ]
DASGRACAS, M [1 ]
HENRIQUES, MO [1 ]
SILVA, PMR [1 ]
MARTINS, MA [1 ]
DOSSANTOS, RR [1 ]
CORDEIRO, RSB [1 ]
机构
[1] FIOCRUZ MS,INST OSWALDO CRUZ,DEPT BIOQUIM & BIOL MOLEC,RIO JANEIRO,BRAZIL
关键词
ENDOTOXIN (LPS); EOSINOPHILS; MACROPHAGES; T LYMPHOCYTES;
D O I
10.1002/jlb.56.2.151
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this study we investigated the involvement of inflammatory cells in the pleural accumulation of eosinophils induced by lipopolysaccharide (LPS). Intrathoracic (i.t.) injection of LPS (250 ng/cavity) into rats induced a significant eosinophil accumulation that developed within 24 h, was maximal at 48 h, and returned to control values within 120 h. This eosinophil influx was preceded by a huge neutrophil influx within 4 h and accompanied by a mononuclear cell accumulation between 24 and 48 h. Pretreatment with an antineutrophil monoclonal antibody (RP-3, 2 ml per animal) selectively reduced the number of circulating neutrophils within 8 h but failed to alter the LPS-induced eosinophilia. Similarly, platelet depletion with an anti-rat platelet antiserum did not alter the LPS-induced eosinophil accumulation. Cyclosporine (50 mg/kg, 12 and 2 h before) partially inhibited (51%) the LPS-induced pleural eosinophilia, whereas the eosinophilia was not changed by prior degranulation of pleural mast cells with polymyxin B (10 mu g/cavity, 24 h before). Moreover, selective depletion of T lymphocytes using an anti-Thy 1.0 monoclonal antibody significantly inhibited the eosinophilia triggered by LPS. The i.t. injection of liposomes containing dichloromethylene diphosphonate significantly reduced (65 %) the number of resident macrophages after 5 days. Under this condition, the eosinophil infiltration induced by LPS was completely inhibited. Accordingly, the i.t. injection of supernatant from macrophage monolayers, obtained from the pleural cavities of LPS-injected rats, into naive recipient animals led to a twofold increase in the number of pleural eosinophils. In conclusion, our data suggest an important role for resident macrophages and T lymphocytes in the eosinophil accumulation induced by LPS.
引用
收藏
页码:151 / 158
页数:8
相关论文
共 37 条
[1]  
AMON EU, 1992, AGENTS ACTIONS, pC250
[2]  
ARENZANASEISDEDOS F, 1985, J IMMUNOL, V134, P2444
[3]  
BOREL JF, 1989, PHARMACOL REV, V41, P259
[4]   LIPOPOLYSACCHARIDE-INDUCED PLEURAL NEUTROPHIL ACCUMULATION DEPENDS ON MARROW NEUTROPHILS AND PLATELET-ACTIVATING-FACTOR [J].
BOZZA, PT ;
CASTROFARIANETO, HC ;
SILVA, AR ;
LARANGEIRA, AP ;
SILVA, PMR ;
MARTINS, MA ;
CORDEIRO, RSB .
EUROPEAN JOURNAL OF PHARMACOLOGY-ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY SECTION, 1994, 270 (2-3) :143-149
[5]   IL-5 ACCOUNTS FOR THE MOUSE PLEURAL EOSINOPHIL ACCUMULATION TRIGGERED BY ANTIGEN BUT NOT BY LPS [J].
BOZZA, PT ;
CASTROFARIANETO, HC ;
PENIDO, C ;
LARANGEIRA, AP ;
SILVA, PMR ;
MARTINS, MA ;
CORDEIRO, RSB .
IMMUNOPHARMACOLOGY, 1994, 27 (02) :131-136
[6]   PHARMACOLOGICAL MODULATION OF LIPOPOLYSACCHARIDE-INDUCED PLEURAL EOSINOPHILIA IN THE RAT - A ROLE FOR A NEWLY GENERATED PROTEIN [J].
BOZZA, PT ;
CASTROFARIANETO, HC ;
MARTINS, MA ;
LARANGEIRA, AP ;
PERALES, JE ;
SILVA, PMRE ;
CORDEIRO, RSB .
EUROPEAN JOURNAL OF PHARMACOLOGY-ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY SECTION, 1993, 248 (01) :41-47
[7]  
BOZZA PT, 1991, BRAZ J MED BIOL RES, V24, P957
[8]   RAT PLEURAL AND PERITONEAL MAST-CELLS STIMULATED AT DIFFERENT CELLULAR-LEVELS - DIFFERENCE IN AND INFLUENCE OF PURIFICATION MEDIA [J].
CABADO, AG ;
VIEYTES, MR ;
BOTANA, LM .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1993, 100 (03) :234-239
[9]  
COLDITZ IG, 1984, J IMMUNOL, V133, P2169
[10]  
COLLINS PD, 1993, IMMUNOLOGY, V79, P312