MONITORING OF A CONJUGATION REACTION BETWEEN FLUORESCEIN ISOTHIOCYANATE AND MYOGLOBIN BY CAPILLARY ZONE ELECTROPHORESIS

被引:28
作者
BANKS, PR
PAQUETTE, DM
机构
[1] Department of Chemistry and Biochemistry, Concordia University, Montreal, Que. H3G 1M8
关键词
D O I
10.1016/0021-9673(94)01110-Z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The conjugation reaction between the amine reactive fluorescent probe fluorescein isothiocyanate and the protein horse heart myoglobin was directly monitored as the reaction progressed by capillary zone electrophoresis with UV absorbance detection. Kinetic analysis of the data indicated that the order of the reaction was first with respect to myoglobin and 1.3 with respect to fluorescein isothiocyanate. The separation of peaks attributable to the incorporation of n=1, 2,...7 fluorochrome labels into the protein has some significance for the generation of single-label protein probes, such as antibodies.
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页码:145 / 154
页数:10
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