ANTIBODY ENGINEERING - ADVANCES FROM THE USE OF ESCHERICHIA-COLI EXPRESSION SYSTEMS

被引:135
作者
PLUCKTHUN, A
机构
[1] Genzentrum der Universität München, C/o Max-Planck-Institut für Biochemic, Martinsried
来源
BIO-TECHNOLOGY | 1991年 / 9卷 / 06期
关键词
D O I
10.1038/nbt0691-545
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Synthesis in Escherichia coli of correctly folded antibody fragments that bind antigen with the same affinity as the whole antibody is now possible. Here I review the techniques for achieving this and the physical properties of the various fragments produced. This technology not only facilitates antibody engineering but is also the basis of screening libraries for binding activity. Although the immunization of animals has not been made unnecessary in the production of monoclonal antibodies, steps toward this goal are now feasible.
引用
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页码:545 / 551
页数:7
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