THE COMPOSITION OF THE INTERSTITIAL FLUID IN THE RETINA OF THE HONEYBEE DRONE - IMPLICATIONS FOR THE SUPPLY OF SUBSTRATES OF ENERGY-METABOLISM FROM BLOOD TO NEURONS

被引:24
作者
CARDINAUD, B
COLES, JA
PERROTTET, P
SPENCER, AJ
OSBORNE, MP
TSACOPOULOS, M
机构
[1] INST ALFRED FESSARD, CNRS, UPR 2212, F-91198 GIF SUR YVETTE, FRANCE
[2] CTR MED UNIV GENEVA, OPHTHALMOL EXPTL LAB, CH-1211 GENEVA 4, SWITZERLAND
[3] UNIV BIRMINGHAM, DEPT PHYSIOL, BIRMINGHAM B15 2TT, ENGLAND
关键词
D O I
10.1098/rspb.1994.0093
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Ion-selective microelectrodes were used to measure extracellular free ion concentrations in the retina of the drone honeybee, Apis mellifera male. Mean values were (in millimoles per litre): Na+, 196; K+, 10.2; Ca2+, 2.0; pH 6.9. The elemental composition of fluid that rose into a micropipette inserted in the retina was obtained by electron microprobe X-ray analysis: from the concentrations of Na and K it was estimated that this fluid was 91% interstitial fluid. Amino acids and carbohydrates were analysed by chromatography. Four amino acids had concentrations > 20 mM: proline (109 mM), glutamine (38 mM) alanine (31 mM) and beta-alanine (24 mM). These concentrations were higher than in the haemolymph. Other amino acids had concentrations of less than 3 mM. The identified carbohydrates were trehalose, glucose, pyruvate and fructose. All of these were less concentrated than in the haemolymph. These results: (ij show that the ion concentrations of previously used Ringer solutions were reasonably correct; (ii) demonstrate properties of the blood-retina barrier; (iii) suggest that the extracellular concentration of alanine is ample for it to serve as a major substrate of neuronal energy metabolism in this tissue.
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页码:49 / 58
页数:10
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