FLUORESCENT IN-SITU HYBRIDIZATION OF A BACTERIAL ARTIFICIAL CHROMOSOME

被引：71

作者：

HANSON, RE ^{[1
]}

ZWICK, MS ^{[1
]}

CHOI, SD ^{[1
]}

ISLAMFARIDI, MN ^{[1
]}

MCKNIGHT, TD ^{[1
]}

WING, RA ^{[1
]}

PRICE, HJ ^{[1
]}

STELLY, DM ^{[1
]}

机构：

[1] TEXAS A&M UNIV,DEPT BIOL,COLLEGE STN,TX 77843

来源：

GENOME | 1995年 / 38卷 / 04期

关键词：

BACTERIAL ARTIFICIAL CHROMOSOME; BAC; GOSSYPIUM; IN SITU HYBRIDIZATION; PHYSICAL MAPPING;

D O I：

10.1139/g95-082

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fluorescent in situ hybridization (FISH) of a 130 kilobase cotton (Gossypium hirsutum L.) bacterial artificial chromosome (BAC) clone containing a high proportion of single-copy DNA produced a large pair of FISH signals on the distal end of the long arm of a pair of chromosomes of the D-genome species G. raimondii Ulbr. and produced a fainter pair of signals on a small submetacentric pair of chromosomes of the A-genome species G. herbaceum L. The signals were syntenic with a nucleolar organizer region in G. raimondii and G. herbaceum. Signal pairs were easily recognized in interphase and metaphase cells either with or without suppression of repetitive sequences with unlabeled G. hirsutum C(0)t-1 DNA. High quality FISH results were consistently obtained and image analysis was not required for viewing or photography. Results indicate that FISH of BAC clones is an excellent tool for the establishment of new molecular cytogenetic markers in plants and will likely prove instrumental in the development of useful physical maps for many economically important crop species.

引用

页码：646 / 651

页数：6

共 21 条

[1] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS [J].