PARTIAL-PURIFICATION OF MYOSIN FROM LILY POLLEN TUBES BY MONITORING WITH INVITRO MOTILITY ASSAY

被引：24

作者：

KOHNO, T

ISHIKAWA, R

NAGATA, T

KOHAMA, K

SHIMMEN, T

机构：

[1] HIMEJI INST TECHNOL,FAC SCI,DEPT LIFE SCI,1479-1 HARIMA SCI PK CITY,HYOGO 67812,JAPAN

[2] UNIV TOKYO,FAC SCI,DEPT BOT,TOKYO 113,JAPAN

[3] GUNMA UNIV,SCH MED,DEPT PHARMACOL,MAEBASHI,GUNMA 371,JAPAN

来源：

PROTOPLASMA | 1992年 / 170卷 / 1-2期

关键词：

ACTIN; ACTOMYOSIN; INVITRO MOTILITY ASSAY; MYOSIN; POLLEN TUBE;

D O I：

10.1007/BF01384459

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Myosin in pollen tubes of Lilium longiflorum was partially purified, using an in vitro motility assay as a monitor. The main components in the partially purified preparation had molecular masses of 110, 120, and 140 kDa in SDS-PAGE. They became bound to actin filaments in an ATP-dependent manner. Among the components, only that of 120 kDa became bound to ATP and was concluded to be the heavy chain of pollen tube myosin.

引用

页码：77 / 85

页数：9

共 35 条

[11] POLLEN-TUBE EXTRACT SUPPORTS THE MOVEMENT OF ACTIN-FILAMENTS INVITRO [J].