THE USE OF TRANSIENT GUS EXPRESSION TO DEVELOP AN AGROBACTERIUM-MEDIATED GENE-TRANSFER SYSTEM FOR KIWIFRUIT

被引:18
作者
JANSSEN, BJ [1 ]
GARDNER, RC [1 ]
机构
[1] UNIV AUCKLAND,CTR GENE TECHNOL,AUCKLAND,NEW ZEALAND
关键词
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have monitored transient GUS expression 4-5 days after cocultivation of leaf explants with Agrobacterium, in order to optimise parameters of cocultivation and so develop an efficient, reproducible gene transfer system in kiwifruit. Factors that were important included the health of the explant, the strain of Agrobacterium, and the binary vector used. Pre-culture of the leaf explants before cocultivation inhibited gene transfer at the cut edge. Placing the explants on moist filter paper during cocultivation gave increased frequencies of gene transfer. Stably transformed, kanamycin-resistant plants were obtained at good frequency from the optimised system. PCR and Southern analysis of the regenerated plants confirmed their transgenic nature.
引用
收藏
页码:28 / 31
页数:4
相关论文
共 35 条
  • [1] An G., 1988, PLANT MOL BIOL MAN A, VA3, P1
  • [2] ATKINSON RG, 1990, NEW ZEAL J CROP HORT, V18, P153
  • [3] AGROBACTERIUM-MEDIATED TRANSFORMATION OF PEPINO AND REGENERATION OF TRANSGENIC PLANTS
    ATKINSON, RG
    GARDNER, RC
    [J]. PLANT CELL REPORTS, 1991, 10 (04) : 208 - 212
  • [4] ATKINSON RG, 1993, PLANT CELL REP, V12, P347, DOI 10.1007/BF00237433
  • [5] CANDY CJ, 1987, THESIS U AUCKLAND NZ
  • [6] CROWHURST RN, 1990, THESIS U AUCKLAND NZ
  • [7] DONG JZ, 1991, PLANT CELL REP, V10, P555, DOI 10.1007/BF00232510
  • [8] EFFICIENT TRANSFER OF A GLYPHOSATE TOLERANCE GENE INTO TOMATO USING A BINARY AGROBACTERIUM-TUMEFACIENS VECTOR
    FILLATTI, JJ
    KISER, J
    ROSE, R
    COMAI, L
    [J]. BIO-TECHNOLOGY, 1987, 5 (07): : 726 - 730
  • [9] AGROBACTERIUM MEDIATED TRANSFORMATION AND REGENERATION OF POPULUS
    FILLATTI, JJ
    SELLMER, J
    MCCOWN, B
    HAISSIG, B
    COMAI, L
    [J]. MOLECULAR & GENERAL GENETICS, 1987, 206 (02): : 192 - 199
  • [10] INVITRO TRANSFORMATION OF PETUNIA CELLS BY AN IMPROVED METHOD OF CO-CULTIVATION WITH A-TUMEFACIENS STRAINS
    FRALEY, RT
    HORSCH, RB
    MATZKE, A
    CHILTON, MD
    CHILTON, WS
    SANDERS, PR
    [J]. PLANT MOLECULAR BIOLOGY, 1984, 3 (06) : 371 - 378