PEAR BLISTER CANKER VIROID IS A MEMBER OF THE APPLE SCAR SKIN SUBGROUP (APSCAVIROIDS) AND ALSO HAS SEQUENCE HOMOLOGY WITH VIROIDS FROM OTHER SUBGROUPS

被引：36

作者：

HERNANDEZ, C

ELENA, SF

MOYA, A

FLORES, R

机构：

[1] CSIC, INST AGROQUIM & TECNOL ALIMENTOS, UNIDAD BIOL MOLEC & CELULAR PLANTAS, CALLE JAIME ROIG 11, E-46010 VALENCIA, SPAIN

[2] UNIV VALENCIA, DEPT GENET & SERV BIOINFORMAT, E-46100 VALENCIA, SPAIN

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-10-2503

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The sequence of pear blister canker viroid (PBCVd), the putative causal agent of pear blister canker (PBC) disease, has been determined. PBCVd consists of a single-stranded circular RNA of 315 nucleotide residues which assumes a branched conformation when it is folded in the model of lowest free energy. PBCVd has highest sequence similarity with grapevine IB viroid (52.4 %), but also contains sequences related to regions present in viroids that belong to different subgroups, suggesting that PBCVd could have developed from RNA recombination between viroids replicating in a common host plant. PBCVd contains almost the entire central sequence which is conserved in the members of the apple scar skin subgroup (apscaviroids) as well as a conserved sequence located in the left-terminal region of apscaviroids and pospiviroids (whose type member is potato spindle tuber viroid). A consensus phylogenetic tree has been obtained in which PBCVd and other viroids previously classified as apscaviroids appear closely related, allowing consideration of PBCVd as a new member of this subgroup.

引用

页码：2503 / 2507

页数：5

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