COMPARISON OF THE AMINO-ACID-SEQUENCES OF 9 DIFFERENT SEROTYPES OF HEPATITIS-B SURFACE-ANTIGEN AND GENOMIC CLASSIFICATION OF THE CORRESPONDING HEPATITIS-B VIRUS-STRAINS

被引:311
作者
NORDER, H
HAMMAS, B
LOFDAHL, S
COUROUCE, AM
MAGNIUS, LO
机构
[1] NATL BACTERIOL LAB, DEPT VIROL, S-10521 STOCKHOLM, SWEDEN
[2] INST NATL TRANSFUS SANGUINE, F-75739 PARIS, FRANCE
[3] NATL BACTERIOL LAB, DEPT BACTERIOL, S-10521 STOCKHOLM, SWEDEN
关键词
D O I
10.1099/0022-1317-73-5-1201
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The surface (S) genes of 12 hepatitis B viruses (HBVs) encoding nine different serotypes of hepatitis B surface antigen (HBsAg) were amplified by the polymerase chain reaction and sequenced. These represented the eight strains of HBV, P1 to P8, defined at an international workshop on HBsAg subtypes in Paris in 1975, and the adrq- subtype. The S genes from additional HBV strains, one ayw4, one adw4 and one ayw1, of sub-Saharan African origin, were also sequenced. The relationship of these 12 new S gene sequences to those of the 20 published previously was investigated by constructing a phylogenetic tree, which confirmed a previous classification into four groups, designated A to D, based on 18 complete HBV genomes. When relating our sequenced S genes to these genomic groups, ayw1 of African origin and P6 (adw2) were both allocated to group A, the reference P1 (ayw1 of Vietnamese origin) was allocated to group B, P5 (ayr), P8 (adr) and adrq- were all related to group C, and P2 (ayw2) and P3 (ayw3) could both be allocated to group D. Interestingly, the S genes of w4 serotype viruses, i.e. P4 (ayw4) and P7 (adw4q-), differed by 4 % or more from both previous groups and from each other, suggesting their classification into two new groups, for which the designations E and F are proposed. Genomes specifying ayw were also found in groups A and B; previously sequenced genomes specifying the ayw subtype have all been confined to group D. There were indications that the epitope for subdeterminants of w resided at amino acid positions 125 to 127. Thus, at positions 125 and 127, ayw1, ayw2 and adw2 had T and P residues, respectively, whereas M and T residues were at the corresponding positions of ayw3. Both ayw4 and adw4 had L at residue 127, and all strains expressing r, apart from P5, had an I instead of a T residue at position 126.
引用
收藏
页码:1201 / 1208
页数:8
相关论文
共 26 条
  • [11] Iswari S. R., 1985, ICMR Annals, V5, P39
  • [12] KOBAYASHI M, 1984, GENE, V30, P227
  • [13] DETECTION OF HEPATITIS-B VIRUS SEQUENCES IN SERUM BY USING INVITRO ENZYMATIC AMPLIFICATION
    LARZUL, D
    GUIGUE, F
    SNINSKY, JJ
    MACK, DH
    BRECHOT, C
    GUESDON, JL
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1988, 20 (03) : 227 - 237
  • [14] HETEROGENEITY OF AUSTRALIA ANTIGEN
    LEBOUVIER, GL
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1971, 123 (06) : 671 - +
  • [15] MAGNIUS L, 1975, ACTA PATH MICRO IM B, V83, P295
  • [16] TYPING OF HEPATITIS-B VIRUS GENOMES BY A SIMPLIFIED POLYMERASE CHAIN-REACTION
    NORDER, H
    HAMMAS, B
    MAGNIUS, LO
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1990, 31 (03) : 215 - 221
  • [17] POINT MUTATION IN THE S-GENE OF HEPATITIS-B VIRUS FOR A D/Y OR W/R SUBTYPIC CHANGE IN 2 BLOOD-DONORS CARRYING A SURFACE-ANTIGEN OF COMPOUND SUBTYPE ADYR OR ADWR
    OKAMOTO, H
    IMAI, M
    TSUDA, F
    TANAKA, T
    MIYAKAWA, Y
    MAYUMI, M
    [J]. JOURNAL OF VIROLOGY, 1987, 61 (10) : 3030 - 3034
  • [18] TYPING HEPATITIS-B VIRUS BY HOMOLOGY IN NUCLEOTIDE-SEQUENCE - COMPARISON OF SURFACE-ANTIGEN SUBTYPES
    OKAMOTO, H
    TSUDA, F
    SAKUGAWA, H
    SASTROSOEWIGNJO, RI
    IMAI, M
    MIYAKAWA, Y
    MAYUMI, M
    [J]. JOURNAL OF GENERAL VIROLOGY, 1988, 69 : 2575 - 2583
  • [19] NUCLEOTIDE-SEQUENCE OF A CLONED HEPATITIS-B VIRUS GENOME, SUBTYPE AYR - COMPARISON WITH GENOMES OF THE OTHER 3 SUBTYPES
    OKAMOTO, H
    IMAI, M
    SHIMOZAKI, M
    HOSHI, Y
    IIZUKA, H
    GOTANDA, T
    TSUDA, F
    MIYAKAWA, Y
    MAYUMI, M
    [J]. JOURNAL OF GENERAL VIROLOGY, 1986, 67 : 2305 - 2314
  • [20] OKAMOTO H, 1987, JPN J EXP MED, V57, P231