GENERATION OF HIGH-DENSITY DNA MARKERS FROM YEAST ARTIFICIAL CHROMOSOME DNA BY SINGLE UNIQUE PRIMER-POLYMERASE CHAIN-REACTION

被引：3

作者：

HADANO, S

ISHIDA, Y

BATES, GP

NAGAYAMA, T

KANAZAWA, I

LEHRACH, H

IKEDA, J

机构：

[1] TOKAI UNIV,SCH MED,ERATO,JRDC,ISEHARA,KANAGAWA 25911,JAPAN

[2] UNIV TOKYO,INST ORTHOPAED SURG,DEPT NEUROL,TOKYO 113,JAPAN

[3] IMPERIAL CANC RES FUND,GENOME ANAL LAB,LONDON WC2A 3PX,ENGLAND

[4] TOKAI UNIV,SCH MED,IKEDA GENOSPHERE PROJECT,ISEHARA,KANAGAWA 25911,JAPAN

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1993年 / 10卷 / 05期

关键词：

D O I：

10.1016/1050-3862(93)90032-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method for the whole sequence amplification of yeast artificial chromosome (YAC) DNA excised from preparative pulsed-field gel electrophoresis using single unique primer-polymerase chain reaction procedures. We used seven contiguous YAC clones, which span 2 Mbp of the Huntington disease gene region on 4p16.3, to amplify the YAC DNAs. The average size of the amplified DNA was approximately 300 bp long, and 12 DNA markers located on the YAC clones positively hybridized with these amplified products, implying that the sequences of the YAC clones were comprehensively amplified by our procedures. These amplified YAC DNAs greatly facilitate the characterization of YAC clones, leading to the detailed analysis of the defined chromosomal region.

引用

收藏

页码：105 / 108

页数：4

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