PCR AMPLIFICATION AND ANALYSIS OF YEAST ARTIFICIAL CHROMOSOMES

被引：13

作者：

SUTCLIFFE, JS

ZHANG, FP

CASKEY, CT

NELSON, DL

WARREN, ST

机构：

[1] EMORY UNIV, SCH MED, HOWARD HUGHES MED INST, ATLANTA, GA 30322 USA

[2] EMORY UNIV, SCH MED, DEPT BIOCHEM, DIV MED GENET, ATLANTA, GA 30322 USA

[3] EMORY UNIV, SCH MED, DEPT PEDIAT, ATLANTA, GA 30322 USA

[4] BAYLOR COLL MED, INST MED GENET, HOUSTON, TX 77030 USA

[5] BAYLOR COLL MED, HOWARD HUGHES MED INST, HOUSTON, TX 77030 USA

来源：

GENOMICS | 1992年 / 13卷 / 04期

关键词：

D O I：

10.1016/0888-7543(92)90051-S

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strategy for the analysis of yeast artificial chromosome (YAC) clones that relies on polymerase chain reaction (PCR) amplification of small restriction fragments from isolated YACs following adapter ligation was developed. Using this method, termed YACadapt, we have amplified several YACs from a human Xq24-qter library and have used the PCR products for physical mapping by somatic cell hybrid deletion analysis and fluorescent in situ hybridization. One YAC, RS46, was mapped to band Xq27.3, near the fragile X mutation. The PCR product is an excellent renewable source of YAC DNA for analyses involving hybridization of YAC inserts to a variety of DNA RNA sources. © 1992.

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页码：1303 / 1306

页数：4

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