FUNCTIONAL-ANALYSIS OF THE PHOSPHOPROTEIN P-II (GLNB GENE-PRODUCT) IN THE CYANOBACTERIUM SYNECHOCOCCUS SP STRAIN PCC-7942

被引:127
作者
FORCHHAMMER, K
DEMARSAC, NT
机构
[1] INST PASTEUR, DEPT BIOCHIM & GENET MOLEC, UNITE PHYSIOL MICROBIENNE, F-75724 PARIS 15, FRANCE
[2] UNIV MUNICH, INST GENET & MICROBIOL, LEHRSTUHL MIKROBIOL, D-80638 MUNICH, GERMANY
关键词
D O I
10.1128/jb.177.8.2033-2040.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The P-II protein (glnB gene product) in the cyanobacterium Synechococcus sp. strain PCC 7942 signals the cellular N status by being phosphorylated or dephosphorylated at a seryl residue. Here we show that the P-II-modifying system responds to the activity of ammonium assimilation via the glutamine synthetase-glutamate synthase pathway and to the state of CO2 fixation. To identify possible functions of P-II in this microorganism, a P-II-deficient mutant was created and its general phenotype was characterized. The analysis shows that the P-II protein interferes with the regulation of enzymes required for nitrogen assimilation, although ammonium repression is still detectable in the P-II-deficient mutant. We suggest that the phosphorylation and dephosphorylation of P-II are part of a complex signal transduction network involved in global nitrogen control in cyanobacteria. In this regulatory process, P-II might be involved in mediating the tight coordination between carbon and nitrogen assimilation.
引用
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页码:2033 / 2040
页数:8
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